Monoclonal Ready-to-Use

Actin, Alpha-Smooth Muscle; 1A4

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	Species: Mouse Monoclonal Clone: 1A4 Isotype: IgG2a,k Species Reactivity: Human, Baboon, Cow, Rabbit, Mouse, Rat, Chicken. Positive Control: Blood vessels Specificity: This antibody recognizes the a-smooth muscle form of actin. It shows no cross reaction with actin from fibroblasts (b- and g-cytoplasmic), striated muscle (a-sarcometric), and myocardium (a-myocardial). Its epitope is composed of the acetyl group and the first 4 amino acids on the N-terminal end of the peptidic chain of a-smooth actin. ScyTek’s 1A4 stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained as they also contain this actin. RUO Datasheet Datasheet European Customers

Actin, Muscle Specific, Clone HHF35

	Species: Mouse Monoclonal Clone: HHF35 Isotype: IgG1, kappa MW: 42kD Species Reactivity: Human Positive Control: Skeletal or cardiac muscle. Specificity: This antibody is specific to alpha and gamma specific actinisomers from skeletal, cardiac and smooth muscle but does not recognize beta and non-smooth muscle gamma actin isomers. In western blot, HHF35 detects a 42kD protein extract from skeletal muscle but not from epithelial cell lines. RUO Datasheet Datasheet European Customers

Actin, Pan; 1A4 + 5C5.F8.C7

	Species: Mouse Monoclonal Clone: 1A4+5C5.F8.C7 Isotype: IgG2ak (1A4) IgMk (5C5.F8.C7) Species Reactivity: Human Positive Contol: Muscle or sarcoma Cellular Localization: Cytoplasmic Specificity: Reacts with µ-smooth muscle as well as a-skeletal and a-cardiac (sarcomeric) isoform of actin. RUO Datasheet Datasheet European Customers

B Lymphocyte Antigen; A27-42

	Species: Mouse Monoclonal Clone: A27-42 Isotype: IgG3,k MW: 36 kD Species Reactivity: Human Specificity: This antibody reacts with a glycoprotein of 36 kD present on the early and activated B cells but does not react with T lymphocytes, granulocytes and macrophages. It reacts with Reed-Sternberg cell in Hodgkin’s lymphoma. Immunoreactivity is confined to the membrane of immunoblasts. RUO Datasheet Datasheet European Customers

Bcl-2-oncoprotein; Clone 124

	Species: Mouse Monoclonal Clone: 124 Isotype: IgG1,kappa MW: 25 kD Species Reactivity: Human Specificity: This antibody reacts with a 25 kD bcl protein, which lies within the cell rather than on the cell surface. It stains neoplastic cells of follicular lymphoma, hairy cell leukaemia, high grade B and T cell lymphomas, lymphoblastic lymphomas and anaplastic large cell lymphoma. RUO Datasheet Datasheet European Customers

Bcl-6; Clone PG-B6P

	Species: Mouse Monoclonal Clone: PG-B6P Isotype: IgG1, kappa Species Reactivity: Human, rabbit, rat, swine, calf, and lamb. Positive Control: Tonsil Cellular Localization: Nuclear Specificity: In normal tonsil the antibody reacts with the nuclei of centroblasts and centrocytes in germinal centers. No reactivity is observed with other germinal center cells, e.g. plasma cells, macrophages, or follicular dendritic cells. Approximately 10% of germinal center T cells are stained with the antibody. The staining pattern of normal spleen B and T cell compartments are similar to that of tonsil. A faint nuclear staining is observed in squamous epithelia in the tonsil, thymus, and skin. In tumor cells the antibody shows strong nuclear labelling of Bcl-6 protein in follicular lymphomas, diffuse large B cell lymphomas, Bufkitt’s lymphomas, and nodular, lymphocyte-predominant Hodgkin’s disease. RUO Datasheet Datasheet European Customers

CA125; Clone Ov185:1

	Species: Mouse Monoclonal Clone: Ov185:1 Isotype: IgG1 Species Reactivity: Human Positive Control: Human Ovary Specificity: Human ovarian cancer antigen (CA125). RUO Datasheet Datasheet European Customers

CA15-3

	Species: Mouse Monoclonal Isotype: IgG1 Species Reactivity: Human Positive Control: Human Breast Specificity: Human Breast Tumor Antigen (CA15-3). No cross-reactivity with Human AFP, CEA, PAP, PSA, CA19-9, or CA125. RUO Datasheet Datasheet European Customers

Cathepsin D; Clone C5

	Species: Mouse Monoclonal Clone: C5 Isotype: IgG2b Species Reactivity: Human Positive Control: Macrophages in breast carcinoma Specificity: Cathepsin D is synthesized as a 54 kD precursor which is proteolytically processed to an intermediate 48 kD single chain which matures into more stable 34 kD and 14 kD two chain form. RUO Datasheet Datasheet European Customers

CD10, CALLA (Neutral Endopeptidase); Clone 56C6

	Species: Mouse Monoclonal Clone: 56C6 Istoype: IgG1 MW: 100kD Species Reactivity: Human, rat. Positive Control: Small intestine or kidney. Specificity: Recognizes a 100kDa glycoprotein, identified as CD10, also known as Commori Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. RUO Datasheet Datasheet European Customers

CD15/Lewis (Reed-Sternberg Cell Marker); Clone FR4A5

	Species: Mouse Monoclonal Clone: FR4A5 Isotype: IgM Specificity: CD15 recognizes a 220kDa carbohydrate structure, which is identified as 3-fucosyl-N-acetyllactosamine (3-FAL) and is also known as CD15 or X-hapten. CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin's disease. CD15 is occassionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. RUO Datasheet Datasheet European Customers

CD20, B-Cell; Clone L26

	Species: Mouse Monoclonal Clone: L26 Isotype: IgG2a,k MW: 33 kD and 30 kD Species Reactivity: Human Positive Control: Tonsil Specificity: This antibody is specific to a 33 kD polypeptide present on the majority of B cells in peripheral blood and lymphoid tissue and also with a minor component of 30 kD. No reactivity with other haematopoietic cells has been observed. RUO Datasheet Datasheet European Customers

CD23, B-Cell; Clone MHM6

	Species: Mouse Clone: MHM6 Isotype: IgG1, Kappa Specificity: In lymphoid tissue, the antibody labels a variable proportion of mantle zone lymphocytes and a fraction of dendritic reticulum cells. In lymph nodes with progressive germinal centre transformation, the number and staining intensity of small follicular lymphocytes are increased. Essentially no staining is seen on splenic marginal zone lymphocytes. The CD23 antigen is expressed on neaoplatic cells from cases of B-Cell chronic lymphocytic leukemia and some cases of centroblastic/centrocytic lymphoma, but not in other types of lymphoid neoplasms. RUO Datasheet Datasheet European Customers

CD3, T-Cell; Clone UCHT1

	Species: Mouse Monoclonal Clone: UCHT1 Isotype: IgG1,k MW: 20 kD Species Reactivity: Human Positive Control: Tonsil Specificity: This antibody reacts with T cells in thymus, bone marrow, and lymphoid tissues. It recognizes non-glycosylated and transmembrane oriented CD3 antigen of molecular weight 20 kD. CD3 is closely associated in the lymphocytes cell surface with the T cell antigen receptors. RUO Datasheet Datasheet European Customers

CD30, Ki-1 Antigen; Clone Ber-H2

	Species: Mouse Monoclonal Clone: Ber-H2 Isotype: IgG1,k MW: 105/120 kD Species Reactivity: Human. Does not react with baboon and monkey Positive Control: Hodgkin’s lymphoma Specificity: Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. The CD30/Ki-1 antigen is expressed by mononuclear Hodgkin and multinucleated Reed-Sternberg cells in Hodgkin’s disease, by the tumor cells of a majority of anaplastic large cell lymphomas, and by a varying proportion of activated T and B cells. RUO Datasheet Datasheet European Customers

CD31, Endothelial Cell; Clone JC/70A

	Species: Mouse Monoclonal Clone: JC/70A Isotype: IgG1,k MW: 100 kD Species Reactivity: Human. Does not react with pig. Positive Control: Blood vessels in skin or intestine Specificity: Recognizes a 100kDa glycoprotein in endothelial cells and 130kDa in platelets. This Mab reacts with endothelial cells in normal tissues and in benign and malignant proliferations. In Cryostat sections and blood smears the antibody also stains megakaryocytes, platelets and occasionally plasma cells. It reacts weakly with mantle zone B cells, peripheral T cells, and neutrophils. RUO Datasheet Datasheet European Customers

CD34, Endothelial Cell; Clone QBEND-10

	Species: Mouse Monoclonal Clone: QBEND-10 Isotype: IgG1,k MW: 110 kD Species Reactivity: Human Positive Control: Normal human skin. Specificity: This antibody reacts with a 110 kD glycoprotein, present on the surface of hematopoietic cells, i.e. myeloid and lymphoid progenitors. The antibody stains vascular endothelial cells. RUO Datasheet Datasheet European Customers

CD43, T-Cell; Clone DF-T1

	Species: Mouse Monoclonal Clone: DF-T1 Isotype: IgG1,k Species Reactivity: Human Positive Control: Tonsil Specificity: This antibody recognizes a 95/115/135kDa (depending on the extent of glycosylation) cell surface glycoprotein, identified as CD43 (leukosialin, sialophorin, or leukocyte sialoglycoprotein). It is expressed on all thymocytes and T-cells. This antibody has been shown useful in identification and classification of T-cell malignacies. RUO Datasheet Datasheet European Customers

CD45, Leucocyte Common Antigen (LCA); Clones PD7/26 and 2B11

	Species: Mouse Monoclonal Clone: PD7/26 and 2B11 Isotype: IgG1,k MW: 180, 190,205 and 220 kD Species Reactivity: Human Positive Control: Tonsil Specificity: The human leucocyte common antigen is a family of five or more high molecular weight glycoproteins of MW 180, 190, 205 and 220 kD, present on the surface of the majority of human leucocytes. This antibody labels lymphoid cells. The antibody labels neoplastic B and T cells in non-Hodgkin’s lymphoma and in leukaemias of B and T cell types. RUO Datasheet Datasheet European Customers

CD45RO, T-Cell; Clone UCHL1

	Species: Mouse Monoclonal Clone: UCHL1 Isotype: IgG2a,k MW: 180 kD Species Reactivity: Human Positive Control: Tonsil Specificity: This antibody reacts with a 180 kD protein, identified as isoform of leucocyte common antigen (CD45RO). The antibody reacts with mature activated T cells, most thymocytes, and a sub-population of resting T cells within both CD4 and CD8 subsets. The antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. RUO Datasheet Datasheet European Customers