Monoclonal Ready-to-Use

CD5 (Mantel Cell Lymphoma Marker); 4C7

Species: Mouse Monoclonal
Clone: 4C7
Isotype: IgG1
Species Reactivity: Human

Specificity: This antibody recognizes a 67kD transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cells. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphoma are CD5-.

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CD74; Clone LN2

Species: Mouse Monoclonal
Clone: LN2
Isotype: IgG1,k
MW: 35 kD
Species Reactivity: Human

Specificity: This antibody reacts with 35 kD antigen present on the nuclear membrane protein related to the invariant chain of the HLA-Dr antigen. This antibody reacts with the germinal center and mantle zone B lymphocytes, interdigitating histiocytes in T cell zones and mononuclear cells and Reed-Sternberg cells in Hodgkin’s disease.

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CD75; Clone LN1

Species: Mouse Monoclonal
Clone: LN1
Isotype: IgM,k
MW: 45-85 kD
Species Reactivity: Human

Specificity: This antibody reacts with 45-85 kD antigen present on the B cells derived from the germinal center. It reacts with the epithelial cells of breast, prostate, kidney, pancreas and lung.

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c-erbB-3 Oncoprotein; Clone RTJ.1

Species: Mouse Monoclonal
Clone: RTJ.1
Isotype: IgM
MW: 160 kD
Species Reactivity: Human

Specificity: This antibody does not cross react with the other members of the type I growth factor receptor family. In human breast carcinoma cell lines, both cytoplasmic and membrane staining has been observed with this antibody. This antibody is useful in the study of c-erbB-2 expression in neoplastic and non-neoplastic diseases.

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Chromogranin A; Clone A3

Species: Mouse Monoclonal
Clone: A3
Isotype: IgG2b,k
Species Reactivity: Human

Specificity: Chromogranin A is a 439 amino acid protein present in secretory granules of a wide variety of endocrine cells and neurons. A positive staining is seen in the secretory granules of parathyroid, adrenal medulla, anterior pituitary gland and Langerhans islets of the pancreas. This antibody stains a variety of neuroendocrine tumors.

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Cyclin D1; Clone P2D11F11

Species: Mouse Monoclonal
Clone: P2D11F11
Isotype: IgG2a
Species Reactivity: Human

Specificity: The cyclin D1 protein belongs to a family of proteins which function primarily in the cell division cycle by regulating the activity of cyclin dependent kinases (cdks). The chromosomal region in which the cyclin D1 gene is located is amplified in a number of cancers. The human cyclin D2 gene has been mapped to the 11q13 locus and has been shown to be the PRAD1 gene product.

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Cyclin D3; Clone DCS-22

Species: Mouse Monoclonal
Clone: DCS-22
Isotype: IgG1
Species Reactivity: Human

Specificity: The human cyclin D3 gene has been mapped to the 6p21 locus. Cyclin D3 binds and activates cdck4. Expression of cyclin D3 occurs late in G1 and unlike cyclins D1 and D2, it is not expressed in macrophages in response to CSF-1 stimulation. Cyclin D3 is regulated by growth factors in other cell types.

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Cytokeratin (45, 56, 56.5); Clone MNF116

Species: Mouse Monoclonal
Clone: MNF116
Isotype: IgG1
MW: 45, 46, 56.5 kD
Species Reactivity: Human

Specificity: It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts and tracheal epithelium.

Cytokeratin 10 (56.5 kD); Clone DE-K10

Species: Mouse Monoclonal
Clone: DE-K10
Isotype: IgG2a
MW: 56.5 kD
Species Reactivity: Human and Dog
Positive Control: Normal skin

Specificity: CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer.

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Cytokeratin 19 (40 kD); Clone BA 17

Species: Mouse Monoclonal
Clone: BA 17
Isotype: IgG1
MW: 40 kD
Species Reactivity: Human

Specificity: It is the smallest human keratin found in most simple and non-keratinizing stratified epithelia. This antigen is not present in adult intrafollicular epidermis. The antibody stains most of the epithelial cell types including ductual and glandular epithelia.

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Cytokeratin 8, Low Molecular Weight; Clone 35bH11

Species: Mouse Monoclonal
Clone: 35bH11
Isotype: IgMk
MW: 54 kD
Species Reactivity: Human, Monkey, Rabbit
Positive Control: Any tissue containing non-squamous epithelium.

Specificity: This antibody strongly stains non-squamous epithelium, but does not react with squamous epithelium. It reacts with the sweat gland and ducts in the skin. Adenocarcinoma of the ovary, gastrointestinal tract, ductal carcinoma of the breast, pancreas and bile ducts, stain positively with this antibody. The antibody does not react with skeletal muscle, nervous tissue or epidermis and mesenchymal tissue such as blood vessels and dermis.

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Cytokeratin AE1 (Acidic); Clone AE1

Species: Mouse Monoclonal
Clone: AE1
Isotype: IgG1
MW: 56.5, 50, 50’, 48 and 40 kD
Species Reactivity: Human, Monkey, Cow, Rabbit, Mouse, Rat, Chicken. Others not tested.
Positive Control: Human epidermal keratin or carcinoma. HT29 cells.

Specificity: Monoclonal antibody AE1 recognizes the 56.5, 50, 50’, 48, and 40kDa keratins of the acidic subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI<5.7) and basic (pI>6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50’, 48, 46, 45, and 40kDa.

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Cytokeratin, High Molecular Weight; Clone 34bE12

Species: Mouse Monoclonal
Clone: 34bE12
Isotype: IgG1k
MW: 68, 58, 56.5 and 50 kD
Species Reactivity: Human, Horse, Monkey, Rabbit
Positive Control: Any tissue containing squamous epithelium.

Specificity: This antibody stains squamous, ductal and other complex epithelium. This antibody reacts with squamous cell and ductal carcinomas of the breast, pancreas, bile duct and salivary gland, in addition to transitional cell carcinomas of the bladder and nasopharnyx, thymomas, and epithelioid mesotheliomas. Mesenchymal tissues such as blood vessel, smooth muscle, skeletal muscle, dermis and nervous tissue are not stained.

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Cytokeratin, High Molecular Weight; Clone AE-3

Species: Mouse Monoclonal
Clone: AE-3
Isotype: IgG1
MW: 58 and 65 to 67 kD
Species Reactivity: Human

Specificity: In keratinized epidermis, 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers. This antibody can be used to stain different cell layers in epithelial tissue and various neoplasms of epithelial origin.

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Cytokeratin, Wide M.W. Range; Clone ST1

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Species: Mouse Monoclonal
Clone: ST1
Isotype: IgG1k
MW: 40-68 kD
Species Reactivity: Human

Specificity: This antibody is used to do research to distinguish epithelial tumors from non-epithelial tumors, and to identify undifferentiated malignant metastatic neoplasms of epithelial origin.

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Desmin, Clone D33

Species: Mouse Monoclonal
Clone: D33
Isotype: IgG1,k
MW: 58 and 65 to 67 kD
Species Reactivity: Human, Baboon, Monkey, Cow, Cat, Dog, Hamster, Rat and Chicken.
Positive Control: Muscle or sarcoma.

Specificity: This antibody is highly specific to desmin and shows no cross-reaction with other intermediate filament proteins. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. It reacts with leiomyosarcomas (smooth muscle) as well as rhabdomyosarcomas (striated muscle).

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Epithelial Membrane Antigen; Clone E29

Species: Mouse Monoclonal
Clone: E29
Isotype: IgG2a
MW: 265-400 kD
Species Reactivity: Human
Positive Control: Breast or colon carcinoma

Specificity: In Western blotting, it recognizes two glycoproteins in MW range of 265-400kDa, identified as epithelial membrane antigen (EMA), or mucin 1 (MUC-1), or polymorphic epithelial mucin (PEM), or sialomucin, or episialin. In immunohistochemical assays, ScyTek’s E29 stains routine formalin/paraffin carcinoma tissues without any special pretreatment. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

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Estrogen Receptor; Clone 1D5

Species: Mouse Monoclonal
Clone: 1D5
Isotype: IgG1,k
MW: 67 kD

Specificity: Recognizes a protein of 67 kDa which is identified as estrogen receptor (ER). Its epitope maps in the N-terminal domain (A/B) of ER. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F.

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Estrogen Receptor; Clone CC4-5

Species: Mouse Monoclonal
Clone: CC4-5
Isotype: IgG1,k
Species Reactivity: Human

Specificity: The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm.

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Factor VIII Related Antigen; Clone F8/86

Species: Mouse Monoclonal
Clone: F8/86
Isotype: IgG1
Species Reactivity: Human

Specificity: Factor VIII is present in endothelial cells, where it shows a granular pattern of reactivity, and in the cytoplasm of megakaryocytic proliferation in human bone marrow samples.

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