Monoclonal Concentrates

Bromodeoxyuridine (BrdUrd); Clone Bu20a, Concentrate

	Species: Mouse Monoclonal Clone: Bu20a Isotype: IgG1,k Species Reactivity: Human Specificity: This antibody is specific to BrdUrd in single-stranded DNA, free BrdUrd or BrdUrd coupled to a protein carrier. It also binds to cells that have incorporated BrdUrd into their DNA during S-phase of the cell cycle. Spec Sheet

Cathepsin D; Clone C5, Concentrate

	Species: Mouse Monoclonal Clone: C5 Isotype: IgG2b Species Reactivity: Human Positive Control: Macrophages in breast carcinoma Specificity: Cathepsin D is synthesized as a 54 kD precursor which is proteolytically processed to an intermediate 48 kD single chain which matures into more stable 34 kD and 14 kD two chain form. Spec Sheet

CD14, Monocyte; Clone TUK4, Concentrate

	Species: Mouse Monoclonal Clone: TÜK4 Isotype: IgG2a MW: 55 kD Species Reactivity: Human Specificity: This antibody reacts with a 55 kD single chain glycoprotein present on the surface of monocyte and macrophages as well as granulocytes, dendritic reticulum cells and Langerhans cells. It stains more than 95% of monocytes and granulocytes in peripheral blood and bone marrow. Spec Sheet

CD15/Lewis (Reed-Sternberg Cell Marker); Clone FR4A5, Concentrate

	Species: Mouse Monoclonal Clone: FR4A5 Isotype: IgM MW: 220 kD Species Reactivity: Human Positive Control: Tonsil Specificity: This antibody recognizes a 220 kD carbohydrate structure which is identified as 3-fucosyl-N-acetyllactosamine (3-FAL) and is also known as CD15 or X-hapten. CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. Spec Sheet

CD2, T-Cell; Clone MT910, Concentrate

	Species: Mouse Monoclonal Clone: MT910 Isotype: IgG1 MW: 46-50 kD Species Reactivity: Human Specificity: This antibody reacts with the E-rosette receptor on T-cells. It is a valuable pan-T antibody, detecting normal T-cells in frozen sections of human tissue and in cell smears. Spec Sheet

CD25, Interleukin 2 Receptor; Clone ACT-1, Concentrate

	Species: Mouse Monoclonal Clone: ACT-1 Isotype: IgG1 MW: 55 kD Species Reactivity: Human Specificity: ACT-1 detects activated lymphoid cells in normal and pathological lymphoid tissue. This antibody labels a varying proportion of lymphoid cells in the T zones and light zones of the germinal centers. It weakly identifies macrophages of germinal centers and macrophages present in the sinuses more strongly. Spec Sheet

CD33, Myeloid Cell; Clone WM-54, Concentrate

	Species: Mouse Monoclonal Clone: WM-54 Isotype: IgG1,k MW: 45 kD Species Reactivity: Human Specificity: This antibody reacts with a 45 kD glycoprotein, present on the surface of mature monocytes. It stains monocytes, granulocytes and bone marrow mononuclear cells. Spec Sheet

CD4, T Cell, Helper/Inducer; Clone MT310, Concentrate

	Species: Mouse Monoclonal Clone: MT310 Isotype: IgG1,k MW: 59 kD Species Reactivity: Human Positive Control: Tonsil Specificity: This antibody reacts with glycoprotein of 59 kD present on most human T cells and is initially co-expressed with CD1 and CD8 antigens on cortical thymocytes. This antibody performs well in cryostat sections and blood smears. Spec Sheet

CD42b, Platelet Glycoprotein; Clone AN51, Concentrate

	Species: Mouse Monoclonal Clone: AN51 Isotype: IgG2a MW: 150 kD Species Reactivity: Human Specificity: This antibody reacts with a glycoprotein of MW 150 kD, present on the surface of megakaryocytes and platelets. The antibody labels megakaryocytes in bone marrow and platelets in peripheral blood and bone marrow. Spec Sheet

CD45RA, B-Cell; Clone 4KB5, Concentrate

	Species: Mouse Monoclonal Clone: 4KB5 Isotype: IgG1,k Species Reactivity: Human Positive Control: Tonsil Specificity: This antibody reacts with most of the B cells in tissue sections and in peripheral blood in addition to a small population of T cells and monocytes. Spec Sheet

CD45RB, Leucocyte Common Antigen (LCA); Clone PD7/26, Concentrate

	Species: Mouse Clone: PD7/26 Isotype: IgG1, Kappa Specificity: This antibody reacts with the epitope encoded by axon B of the CD45 gene. The antibody stains B cells, subsets of T cells, monocytes, and granulocytes. Spec Sheet

CD45RO, T-Cell; Clone UCHL1, Concentrate

	Species: Mouse Monoclonal Clone: UCHL1 Isotype: IgG2a,k MW: 180 kD Species Reactivity: Human Positive Control: Tonsil Specificity: This antibody reacts with a 180 kD protein, identified as isoform of leucocyte common antigen (CD45RO). The antibody reacts with mature activated T cells, most thymocytes, and a sub-population of resting T cells within both CD4 and CD8 subsets. The antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Spec Sheet

CD61, Platelet Glycoprotein IIIa; Clone Y2/51, Concentrate

	Species: Mouse Monoclonal Clone: Y2/51 Isotype: IgG1 MW: 110 kD Species Reactivity: Human Specificity: This antibody recognizes a glycoprotein of 110 kD molecular weight present on human platelets and also reacts with megakaryocytes and megakaryoblasts. The antigen is also present on endothelium and osteoclasts. The glycoprotein IIIa molecule produced by endothelium cells is identical in sequence to platelet glycoprotein IIIa. Spec Sheet

CD68, Macrophage; Clone EMB11, Concentrate

	Species: Mouse Monoclonal Clone: EMB11 Isotype: IgG3 MW: 110 kD Species Reactivity: Human Specificity: This antibody reacts with a glycoprotein of 110 kD, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. The PG-M1 differ from EMB11 (CD68) because of its non-reactivity with granulocytes and their precursor cells. Spec Sheet

CD7, T-Cell; Clone DK24, Concentrate

	Species: Mouse Monoclonal Clone: DK24 Isotype: IgG2b, k MW: 41 kD Species Reactivity: Human Specificity: This antibody reacts with a glycoprotein of 41 kD present on the surface of the majority of human T cells. It stains most of the cortical thymocytes and also medullary thymocytes. Spec Sheet

CD8, T-Cell, Supressor; Clone DK25, Concentrate

	Species: Mouse Monoclonal Clone: DK25 Isotype: IgG1,k MW: 32 kD Species Reactivity: Human Positive Control: Tonsil Specificity: This ScyTek antibody labels suppressor/cytotoxic T cells in peripheral human lymphoid tissue. These cells constitute only 15-20% of T cells in T cell regions (e.g. interfollicular areas) accounting for an even lower percentage of T cells within germinal centers. Conversely, up to 80% of T cells in bone marrow and gut epithelium are of suppressor/cytotoxic type and can thus be labeled with this antibody. In human thymus the antibody labels the great majority of cortical thymocytes and approximately 30% of medullary thymocytes. In human spleen it stains T cells found in the sinus lining in addition to suppressor/cytotoxic T cells. Spec Sheet

Cortical Thymocyte, CD1a: Clone NA1/34, Concentrate

	Species: Mouse Monoclonal Clone: NA1/34 Isotype: IgG2a MW: 49 kD Species Reactivity: Human Specificity: This antibody reacts with the largest of the three heavy chains (MW 49 kD) of CD1 antigen. It reacts with human cortical thymocytes but shows little or no reactivity with medullary thymocytes and peripheral T cells. Spec Sheet

Cytokeratin 10 (56.5 kD); Clone DE-K10, Concentrate

	Species: Mouse Monoclonal Clone: DE-K10 Isotype: IgG2a MW: 56.5 kD Species Reactivity: Human and Dog Positive Control: Normal skin Specificity: CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. Spec Sheet

Cytokeratin 10/13 (53 & 56.5 kD); Clone DE-K13, Concentrate

	Species: Mouse Monoclonal Clone: DE-K13 Isotype: IgG2a MW: 53 & 56.5 kD Species Reactivity: Human and Cat Positive Control: Tongue or esophagus Specificity: This antibody recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections DE-K13 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Spec Sheet

Cytokeratin 18; Clone DC10, Concentrate

	Species: Mouse Monoclonal Clone: DC10 Isotype: IgG1 MW: 45 kD Species Reactivity: Human Positive Control: Skin or hepatocellular carcinoma. Specificity: Recognizes a single polypeptide of 45kDa, identified as keratin 18 which belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 8. This antibody recognizes a variety of simple epithelia including glandular epithelium but not stratified squamous epithelia. It was reported that tissues from gastrointestinal tract are positive for both keratin 8 and 18 but do not contain keratin 14. Spec Sheet