Monoclonal Concentrates

Cytokeratin AE1 (Acidic); Clone AE1, Concentrate

	Species: Mouse Monoclonal Clone: AE1 Isotype: IgG1 MW: 56.5, 50, 50’, 48 and 40 kD Species Reactivity: Human, Monkey, Cow, Rabbit, Mouse, Rat, Chicken. Others not tested. Positive Control: Human epidermal keratin or carcinoma. HT29 cells. Specificity: Monoclonal antibody AE1 recognizes the 56.5, 50, 50’, 48, and 40kDa keratins of the acidic subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI<5.7) and basic (pI>6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50’, 48, 46, 45, and 40kDa. Spec Sheet

Epidermal Growth Factor Receptor (EGFR); Clone EGFR.1 Concentrate

	Species: Mouse Monoclonal Clone: EGFR.1 Isotype: IgG2b,k MW: 170 kD Species Reactivity: Human and Horse. Does not react with mouse and rat. Positive Control: A431 cells express only the intact and not the vIII variant of EGFR. HC2 20 d2 cells express the vIII variant. In normal breast, ducts and lobules show diffuse cytoplasmic staining. Specificity: It recognizes a 170kDa protein, identified as the first member (EGF-Receptor) of the type I family of growth factor receptors. It does not react with vIII variant of EGFR suggesting that its epitope must map between a 6-273 in the extracellular domain of EGFR. Does not interfere with the binding of EGF to EGFR and shows no cross-reaction with erbB-2, erbB-3, or erbB-4. It is particularly well suited for immunoprecipitating the unoccupied as well as the occupied EGFR. Spec Sheet

GFAP - Glial Fibrillary Acidic Protein; Clone GA5, Concentrate

	Species: Mouse Monoclonal Clone: GA5 Isotype: IgG1,k MW: 52 kD Species Reactivity: Human Specificity: This antibody reacts with the 52 kD intermediate filament protein GFAP in brain and spinal cord. It labels some astrocytes and some CNS ependymal cells but not oligodendrocytes or neurons. This antibody does not react with other intermediate filament proteins. Spec Sheet

Glycophorin C; Clone Ret40f, Concentrate

	Species: Mouse Monoclonal Clone: Ret40f Isotype: IgG1 Species Reactivity: Human Specificity: This antibody recognizes sialo-glycophorin beta, present on the human erythrocyte membrane. It labels all the red blood cells and their precursors but not lymphocytes. Spec Sheet

HLA-DR, DP, DQ, DX; Clone CR3/43, Concentrate

	Species: Mouse Monoclonal Clone: CR3/43 Isotype: IgG1 MW: 28 kD Species Reactivity: Human Specificity: This antibody reacts with the beta chain of all products of the gene subregions DP, DQ, and DR. It stains B cells, interdigitating reticulum cells, Langerhan’s cells and many macrophages. This antibody does not react with normal T cell and polymorphs but stains activated T cells. Spec Sheet

Ki-67 Antigen; Clone Ki-67, Concentrate

	Species: Mouse Monoclonal Clone: Ki-67 Isotype: IgG1 Species Reactivity: Human Specificity: Ki-67 labels proliferating cells in cryostat sections of all human tissues and reacts with cells at all stages of the cell cycle (late G1, S, G2 and M), but not with cells in G0 phase. The reaction with this antibody serves as an indicator of the growth fraction; that is the number of cells undergoing active division. Spec Sheet

p105; Clone 780-3.9, Concentrate

	Species: Mouse Monoclonal Clone: 780-3.9 Isotype: IgM Species Reactivity: Human Specificity: The p105 antigen is localized in the interchromatin granules. Its level increases during G1 phase until G2 phase. In studies involving lymphomas and tumors of the brain and colon, p105 positivity has been found to correlate with mitotic activity and the degree of anaplasia. Spec Sheet

p21ras; Clone NCC-RAS-001, Concentrate

	Species: Mouse Monoclonal Clone: NCC-RAS-001 Isotype: IgG1 MW: 21 kD Species Reactivity: Human Specificity: Physiologically, p21ras proteins have been demonstrated to be critical in signal transduction, playing an essential role in orderly cell growth, development and enzyme synthesis, as well as cell induction. This antibody recognizes an epitope within the amino acid region 126-140 at the carboxy terminal part of p21H-ras. Significantly greater reaction may be expected with p21 from wild type and mutants of H-ras than with the proteins encoded by K- and N-ras. Spec Sheet

Sialosyl-Tn Antigen; Clone HB STn1, Concentrate

	Species: Mouse Monoclonal Clone: HB STn1 Isotype: IgG1, k Species Reactivity: Human Specificity: Glycosylation of carbohydrate structures during the synthesis of O-linked oligosaccharides is involved in the pathogenesis and progression of a number of tumor types. Since antibodies against Tn-antigen, sialosyl-Tn antigen and Thomsen-Freidenreich antigen are used to identify these specific carbohydrate derivatives, the antibodies may be useful in the evaluation of these pathologic states. Sialosyl-Tn antigen demonstrates restricted expression in normal and hyperplastic tissue is present in high proportion in epithelial carcinoma cells. It has equivalent specificity to the antibody B72.3 (anti-tumor associated glycoprotein, TAG-72) and TKH2. Spec Sheet

Thyroglobulin; Clone DAK-Tg6, Concentrate

	Species: Mouse Monoclonal Clone: DAK-Tg6 Isotype: IgG2a MW: 300 kD Species Reactivity: Human Specificity: Reacts with cells in both normal, hyperplastic, and neoplastic thyroids. The antibody can be used on both cryostat and formalin-fixed, paraffin-embedded tissue sections. Spec Sheet