Precipitating

AEC Chromogen/Substrate Bulk Kit

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Is a widely used chromogen for immunohistochemical staining and immunoblotting. AEC used with the peroxidase enzyme produces a red end product that is soluble in alcohol and must be used with an aqueous mounting media. This product is available in a two component form.
Spec Sheet

Alk-Phos Blue Kit

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Alk-Phos Blue is a chromogen for immunohistochemical staining and immunoblotting. When in the presence of alkaline phosphatase enzyme, Alk-Phos Blue produces a bright blue precipitate that may be permanently mounted with minimal loss of intensity.
Spec Sheet

BCIP/INT Solution

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5-Bromo-4-Chloro-3-IndolylPhosphate/p-Iodonitrotetrazolium (BCIP/INT) Solution
Is a chromogen used in immunohistochemical and in-situ staining. This reagent is available as a single component, ready-to-use reagent. When in the presence of the Alkaline-Phosphatase enzyme, BCIP/INT produces a brown precipitate that is soluble in alcohol.
Spec Sheet

BCIP/NBT Solution

5-Bromo-4-Chloro-3-Indolyl Phosphate/nitroblue Tetrazolium (BCIP/NBT) Solution
Is a widely used chromogen for immunohistochemical and in-situ staining. This reagent is available as a single component, ready-to-use reagent. When in the presence of the Alkaline-Phosphatase enzyme, BCIP/NBT produces a purple precipitate that is soluble in alcohol.
Spec Sheet

DAB Chromogen/Substrate Bulk pack

3,3'-Diaminobenzidine (DAB) Ready-to-Use
Is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of the peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. Stained slides may be mounted in any commercially available permanent mounting media. This product is available in a convenient two component form.
Spec Sheet

DAB Chromogen/Substrate Bulk Pack (High Contrast)

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This product has been developed for applications that require high contrast between the chromogen and Hematoxylin counterstain. The resulting stain is a darker brown than standard DAB and somewhat more sensitive. ScyTek recommends using Hematoxyling for Automation (catalog # HAQ500) for optimal contrast. 3,3'Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate (High Contrast). The standard working dilution is 50ul (0.9mg) of DAB Chromogen per 1ml of DAB Substrate (High Contrast), although the ratio can be adjusted as desired. The use of liquid components reduces some risks associated with handling powders (ie. dust inhalation), and eliminates waste which often results from using tablets that require a predetermined final volume. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.
Spec Sheet

DAB One-Component Solution (3,3'-Diaminobenzidine)

ScyTek Laboratories, Inc. introduces the first functional single component DAB reagent on the market. All mixing and waste is eliminated using ScyTek's new highly sensitive DAB one-component reagent. This reagent produces a crisp, clean, very intense stain that is free of unwanted background. When in the presence of peroxidase, DAB produces a brown precipitate that is insoluble in alcohol. Stained slides may be mounted in any commercially available mounting media. This product is stable for 2-3 months.
Spec Sheet

Fast-Red Substrate Pack

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Is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of the Alkaline-Phosphatase enzyme, Fast-Red produces a bright red precipitate that is soluble in alcohol and must be used with an aqueous mounting media (AMT030). For convenience, Fast-Red is available in a convenient pack or in economical larger volumes (1 tablet dissolved in 5ml of buffer).

Substrate Pack: 11 Fast-Red Tablets
55ml Naphthol Phosphate Buffer (5 x 11)
Spec Sheet

New Fuchsin Kit

This is a chromogen for immunohistochemical staining and immunoblotting. When in the presence of alkaline phosphatase enzyme, New Fuchsin produces an intense red/fuchsia precipitate that may be permanently mounted.

Pack Includes:

64 ml 60ml Buffer
2ml New Fuchsin Concentrate
2ml Activator

430 ml 400ml Buffer
15ml New Fuchsin
15ml Activator

4,250 ml 4,000ml Buffer
125ml New Fuchsin
125ml Activator
Spec Sheet

TMB Precipitating (High Sensitivity)

This liquid substrate for peroxidase consists of tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in a single-reagent stabilized form. The reagent has been specifically formulated for measuring peroxidase in EIA systems. This reagent is stable for long-term storage, and provides sensitivity equal to, or greater than, that of other commercially available chromogens.
Spec Sheet

TMB Precipitating (Standard Sensitivity)

This liquid substrate for peroxidase consists of tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in a single-reagent stabilized form. The reagent has been specifically formulated for measuring peroxidase in EIA systems. This reagent is stable for long-term storage, and provides sensitivity equal to, or greater than, that of other commercially available chromogens.
Spec Sheet

TMB Precipitating Reagent (For Membranes)

This liquid substrate for peroxidase consists of tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in a single-reagent stabilized form. The reagent has been specifically formulated for measuring peroxidase on membranes and other surfaces. When reacted with peroxidase, the result is an insoluble blue reaction product. This reagent is not designed for use in microwell applications.