HRP Kits

AEC Chromogen/Substrate Bulk Kit

Larger picture

click ... Send me a sample and a personalized quote.

Is a widely used chromogen for immunohistochemical staining and immunoblotting. AEC used with the peroxidase enzyme produces a red end product that is soluble in alcohol and must be used with an aqueous mounting media. This product is available in a two component form.
Spec Sheet

AEC Chromogen/Substrate Kit

Is a widely used chromogen for immunohistochemical staining and immunoblotting. AEC used with the peroxidase enzyme produces a red end product that is soluble in alcohol and must be used with an aqueous mounting media. This product is available in a two component form. For maximum flexibility, this product is available in bulk amounts or convenient dropper-top Substrate Packs.

Substrate Pack:
55ml Substrate (5 x 11 vials)
3ml AEC Chromogen
Spec Sheet

DAB (Post) Enhancing Solution

This single component reagent is designed to be used in a post-staining procedure. After normal DAB development, if additional sensitivity is needed, the slides are simply dipped in this reagent for 1-2 minutes.
Spec Sheet

DAB Away Kit

Contamination with Diaminobenzidine residue is a problem seen in many laboratories. Of particular concern to the technician is the reusable glassware and staining pads. This product completely removes the stain, rather than simply reduce the color intensity as the use of bleach does. The three component kit makes one liter of cleaning solution.

COMPONENTS:
DAB Reagent #1 - 125 ml
DAB Reagent #2 - 125 ml
Decolorizer - 250 ml
Spec Sheet

DAB Chromogen/Substrate Bulk pack

3,3'-Diaminobenzidine (DAB) Ready-to-Use
Is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of the peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. Stained slides may be mounted in any commercially available permanent mounting media. This product is available in a convenient two component form.
Spec Sheet

DAB Chromogen/Substrate Bulk Pack (High Contrast)

Larger picture

click ... Send me a sample and a personalized quote.

This product has been developed for applications that require high contrast between the chromogen and Hematoxylin counterstain. The resulting stain is a darker brown than standard DAB and somewhat more sensitive. ScyTek recommends using Hematoxyling for Automation (catalog # HAQ500) for optimal contrast. 3,3'Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate (High Contrast). The standard working dilution is 50ul (0.9mg) of DAB Chromogen per 1ml of DAB Substrate (High Contrast), although the ratio can be adjusted as desired. The use of liquid components reduces some risks associated with handling powders (ie. dust inhalation), and eliminates waste which often results from using tablets that require a predetermined final volume. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.
Spec Sheet

DAB Chromogen/Substrate Kit

3,3'-Diaminobenzidine (DAB) Ready-to-Use
Is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of the peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. Stained slides may be mounted in any commercially available permanent mounting media. This product is available in a convenient two component form. For maximum flexibility, this product is available in bulk amounts or convenient dropper-top substrate packs.

Substrate Pack:
55ml DAB Substrate (x11 vials)
3ml DAB Chromogen
Spec Sheet

DAB Chromogen/Substrate Kit (High Contrast)

Larger picture

This product has been developed for applications that require high contrast between the chromogen and Hematoxylin counterstain. The resulting stain is a darker brown than standard DAB and somewhat more sensitive. ScyTek recommends using Hematoxyling for Automation (catalog # HAQ500) for optimal contrast. 3,3'Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate (High Contrast). The standard working dilution is 50ul (0.9mg) of DAB Chromogen per 1ml of DAB Substrate (High Contrast), although the ratio can be adjusted as desired. The use of liquid components reduces some risks associated with handling powders (ie. dust inhalation), and eliminates waste which often results from using tablets that require a predetermined final volume. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.
Spec Sheet

DAB Differentiating Solution

May be used for removal of excess DAB staining from tissue specimens.
Spec Sheet

DAB Enhancer Solution

Is a single component reagent that is added to the DAB solution prior to incubation on the tissue section. One drop (50 microliters) of DAB Enhancer is added to each one ml of DAB Substrate.
Spec Sheet

DAB One-Component Solution (3,3'-Diaminobenzidine)

ScyTek Laboratories, Inc. introduces the first functional single component DAB reagent on the market. All mixing and waste is eliminated using ScyTek's new highly sensitive DAB one-component reagent. This reagent produces a crisp, clean, very intense stain that is free of unwanted background. When in the presence of peroxidase, DAB produces a brown precipitate that is insoluble in alcohol. Stained slides may be mounted in any commercially available mounting media. This product is stable for 2-3 months.
Spec Sheet