Ancillary Reagents

CSC Attachment Factor

Attachment Factor is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Attachment Factor also contributes to correct in vitro growth factor and biological response modifier presentation. Use of Attachment Factor is critically important when cultures are to be initiated, grown, passaged, or used within a Serum-Free Medium.

The biologically active elements of Attachment Factor are derived by a chromatographics process from heat-inactivated charcoal-stripped bovine serum, and delivered at a 1X in an Earle’s salt-based HEPES-buffered collagen vehicle. ScyTek exclusively uses and strongly recommends the use of serum products 100% traceable to cattle processed in the USA at USDA inspected facilities.

Attachment Factor is complementary to the Passage Reagent Group (Catalog # CSC005), a matched set of reagents for transferring, passaging, and freezing cell cultures.

Attachment Factor is qualified for use with most suspension and anchorage dependent human and animal primary an established cell lines. Use of Attachment Factor improves plating efficiency, shortens lag time after passage, enhances growth rates, and promotes the organization of polarity and cytoskeletal architecture, and can dramatically increase cell yield during cell culture expansion.
Spec Sheet

CSC Cryopreservation Medium (Formulated with Serum)

Cryopreservation Medium contains conditioned Medium and DMSO, and is ready to thaw and use as delivered.

Use of the Passage Reagent Group (catalog# CSC005) to release cells from culture for freezing is critically important to minimizing membrane and cytoskeletal damage. Cell membranes are materially and cumulatively damaged by detachment or when subjected to the stress of freezing and thawing. Use of Passage Reagent Group (catalog# CSC005) in conjunction with Cryopreservation Medium and Attachment Factor (catalog# CSC001) minimizes cellular stress and damage associated with these processes and result in a much better yield.
Spec Sheet

CSC Growth Factor

Growth Factor is the broad-spectrum mitogen in our Complete Medium and Complete Serum-Free Medium. Growth Factor is acidic fibroblast growth factor (aFGF) and Porcine Heparin formulated at 100X in dPBS.

Growth Factor is purified from bovine hypothalamus and is 100% traceable to cattle processed in the USA at USDA inspected facilities. Working concentration is 50µg/ml. Biological activity is adjusted to 110% of maximum using 2V0-C25 human venous cells and ACBRI 181 and 376 Primary Mv Cells.

Growth Factor is qualified for use when initiating, thawing, expanding, or passaging cultures of mesenchymal-derived cells in either suspension or monolayer cultures and is included as part of all formulations of our Complete Medium Kits. Growth Factor (5ml) prepares 500ml medium at 1X (final concentration).

Growth Factor is qualified for use with our Media, with most classical media, and with Attachment Factor, Passage Reagent Group, and Cryopreservation Medium for the culture of most human and animal primary cell cultures and cell lines.
Spec Sheet

CSC Passage Reagent Group

The Passage Reagent Group (PRG) is a matched set of CSC Certified reagents (100ml each) for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1 (EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution) and PRG-3 (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-a prepares for PRG-2, which contains highly purified trypsin. PRG-3 inactivates the protease in PRG-2 and stabilizes the cell membranes.

Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the PRG greatly minimizes damage and stress to cells during passage or freezing of cell cultures.

The PRG-2 formulation allows a very substantial reduction (<40%) in the amount of Trypsin (BAEE units/ml) required to detach cells compared to typical commercial trypsin solutions.The trypsin in the PRG-2 is stoiciometrically inactivated by trypsin inhibitors in the PRG-3 formulation, preventing nonspecific protease damage to cell membranes after detachment.PRG-3 and Attachment Factor are engineered to work together to stabilize the cell membrane and combine to promote spreading and the establishment of correct polarity after subculture.The PRG is qualified for use with all Certified Media: use of PRG and Attachment Factor are absolutely critical for cells to be passaged or frozen in a Serum-Free Medium System.Spec Sheet

CSC Serum-Free Cryopreservation Medium

Serum-Free Cryopreservation Medium contains conditioned Medium and DMSO, and is ready to thaw and use as delivered.

Use of the Passage Reagent Group (catalog# CSC005) to release cells from culture for freezing is critically important to minimizing membrane and cytoskeletal damage. Cell membranes are materially and cumulatively damaged by detachment or when subjected to the stress of freezing and thawing. Use of Passage Reagent Group (catalog# CSC005) in conjunction with Cryopreservation Medium and Attachment Factor (catalog# CSC001) minimizes cellular stress and damage associated with these processes and result in a much better yield.

Correct use of the Passage Reagent Group (catalog# CSC005), Serum-Free Cryopreservation Medium and Attachment Factor (catalog# CSC001) are absolutely critical for freezing and recovering cells cultured in a Serum-Free Medium system.
Spec Sheet