Epithelial Membrane Antigen; Clone E29 (Concentrate)
Immunogen: Delipidated extract of human milk fat globule membranes
Isotype: IgG2a, kappa
Species Reactivity: Human. Reacts moderately with Pig and Dog. Others not known.
Positive Control: MCF-7 or MDA-231 cells. Breast or colon carcinoma.
Specificity: In Western blotting, this antibody recognizes proteins in a MW range of 265-400kDa, identified as different glycoforms of EMA (MUC1). This antibody reacts with the DTRP epitope within the tandem repeats. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. An antibody to EMA (MUC1) is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.