Mouse to Mouse HRP (DAB) Staining System
Mouse to Mouse HRP (DAB) Staining System is specially designed for using mouse primary antibodies on mouse tissues with minimum cross-reactivity to endogenous mouse IgG. ScyTek's Mouse to Mouse HRP (DAB) Staining System provides unmatched sensitivity with incubation times of 10 minutes each for the Link Antibody and Enzyme Label. This kit includes our AEC chromogen, which can be cover slipped with solvent based mounting media for long term storage.
Incubation with an unconjugated primary antibody specific to the target antigen.
Apply Peroxide Block and incubate for 10 min.
Apply Super Block and incubate for 5 min.
Apply Mouse-to-Mouse Blocking Reagent for 10-60 min.
Apply primary antibody and incubate.
Apply UltraTek Anti-Polyvalent.
Appy UltraTek HRP.
Apply DAB Chomogen/Substrate for 10-20 min.
Peroxide Block - 1 x 8ml - ACA008
Super Block - 1 x 8ml - AAA008
Mouse-to-Mouse Blocking Reagent - 1 x 8ml - MTM008
UltraTek Anti-Polyvalent - 1 x 8 ml - ABN008
UltraTek HRP - 1 x 8ml - ABL008
DAB Chromogen Concentrate - 1 x 3ml - ACB003
DAB Substrate Buffer - 6 x 5ml - ACC005