Mouse to Mouse Alk-Phos (Permanent Red) Staining System
Mouse to Mouse Alk-Phos (Permanent Red) Staining System is specially designed for using mouse primary antibodies on mouse tissues with minimum cross-reactivity to endogenous mouse IgG. ScyTek's Mouse to Mouse Alk-Phos (Permanent Red) Staining System provides unmatched sensitivity with incubation times of 10 minutes each for the Link Antibody and Enzyme Label. This kit includes our Permanent Red chromogen, which can be cover slipped with solvent based mounting media for long term storage.
Incubation with an unconjugated primary antibody specific to the target antigen.
Apply Super Block and incubate for 5 min.
Apply Mouse-to-Mouse Blocking Reagent for 10-60min.
Apply primary antibody and incubate.
Apply UltraTek Anti-Polyvalent.
Apply UltraTek Alk-Phos.
Apply Permanent Red for 10-20 min.
Super Block - 1 x 8ml - AAA008
Mouse-to-Mouse Blocking Reagent - 1 x 8ml - MTM008
UltraTek Anti-Polyvalent - 1 x 8 ml - ABN008
UltraTek Alkaline Phosphatase - 1 x 8ml - ABM008
Permanent Red Concentrate - 1 x 2ml - PRC002
Permanent Red Buffer - 7 x 5ml - PRB005