The UltraTek line is our leading edge system, designed to provide optimal staining with incubation times of 10 minutes each for the link antibody and enzyme label. UltraTek Anti-Mouse is specially formulated and optimized to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique requires the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. For most procedures, commercially available primary antibodies can be diluted up to 50% more than with other systems.
Incubate on tissue section after application of the primary antibody.