Permanent Mounting Media (Aqueous)
AEC and Fast Red are common chromogens for peroxidase and alkaline phosphatase based immunostaining. Slides stained with these chromogens cannot be stored permanently. Permanent Mounting Medium (Aqueous) has been designed to overcome this limitation. This product is an aqueous mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without fading of the chromogens. Due to the superior refractive index, tissues mounted in this medium look like dehydrated specimens. No cover slipping is required. However, if cover slipping is desired, dry slides can be post mounted using an organic based mounting medium. Advantages of this product include: no coverslip, no exposure to the organic fumes, permanent storage of slides and high resolution of tissue specimens. This reagent is compatible with AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. High pH ensures increased stability of fluorescence.
Apply 2-3 drops directly on tissue sections. Gently rotate glass slide to make a thin layer of medium. Make certain that tissue is completely covered.