UltraTek Horseradish Peroxidase
The UltraTek line is our leading edge system, designed to provide optimal staining with incubation times of 10 minutes each for the link antibody and enzyme label. UltraTek Horseradish Peroxidase is specially formulated and optimized to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique requires the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. For most procedures, commercially available primary antibodies can be diluted up to 50% more than with other systems.
Summary Protocol:
Incubate on tissue section after application of the primary antibody.