UltraTek HRP Anti-Polyvalent (AEC) Staining System
UltraTek HRP Anti-Polyvalent (AEC) Staining System, AMG080, 70 slides, is a complete kit to detect primary antibody with mouse and/or rabbit origin by using an HRP-labeled-streptavidin and a biotinylated anti-mouse/rabbit secondary antibody.
The complete ScyTek kit is supplied with full instructions for use and supplied with ScyTek's proprietary formulations of peroxidase block (ACA) and Super Block (AAA) a protein blocking reagent, a biotinylated secondary antibody UltraTek Anti-Polyvalent (ABN), UltraTek Horseradish Peroxidase (ABL) streptavidin-HRP and AEC Chromogen/Substrate for a crisp clear red final stain.
Summary Protocol:
Incubation with an unconjugated primary antibody specific to the target antigen.
The primary antibody is bound to the biotinylated secondary antibody.
The secondary antibody is then bound to the HRP-Labeled streptavidin.
A red color is created when the HRP is bound to site of the primary antibody by reacting with AEC.
Kit Components:
Peroxide Block - 1 x 8ml - ACA008
Super Block - 1 x 8ml - AAA008
UltraTek Anti-Polyvalent - 1 x 8ml - ABN008
UltraTek HRP - 1 x 8 ml - ABL008
AEC Chromogen - 1 x 3ml - ACD003
AEC Substrate - 7 x 5ml - ACE005
Alternate streptavidin-biotin IHC detection kits:
Anti-Mouse HRP-AEC IHC kit UltraTek HRP Anti-Mouse (AEC) Staining System - AKB080.
Anti-Rabbit HRP-AEC IHC Kit - UltraTek HRP Anti-Rabbit (AEC) Staining System - AKD080.
HRP Anti-Polyvalent (DAB) Staining System - UltraTek HRP Anti-Polyvalent (DAB) Staining System - AMF080.
For high sensitivity HRP-Polymer IHC detection kits:
Anti-Mouse / Anti-Rabbit HRP-DAB Polymer IHC Kit - CRF Anti-Polyvalent HRP Polymer (DAB) Stain Kit - CPH080.
For AEC Chromogen detection kits see UltraTek HRP AEC Staining Systems.