DAB (Post) Enhancing Solution
3,3'Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. This product is available in a two-component form which consists of a liquid, refrigerator stable DAB Chromogen and DAB Substrate. DAB (Post) Enhancing Solution has been developed to enhance DAB staining in a post staining procedure. The DAB will turn a darker and more intense brown after post-staining treatment.
Items recommended but not included:
DAB Substrate
DAB Chromogen
Summary Protocol:
Completely cover tissue section and incubate for 1-2 minutes.