Carcinoembryonic Antigen (CEA) / CD66; Clone C66/1291
Species: Mouse
Immunogen: Recombinant full-length human CEA protein
Clone: C66/1291
Isotype: IgG1
Entrez Gene ID: 1048
Hu Chromosome Loc.: 19q13.2
Synonyms: Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, Meconium antigen 100, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)
Mol. Weight of Antigen: 80-200kDa
Format: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM
PBS with 0.05% BSA & 0.05% azide.
Specificity: This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family.
Background: CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
Species Reactivity: Human
Positive Control: MCF7 or 293T cells. Human colon carcinoma.
Cellular Localization: Apical cell membrane, Cell membrane, Cell surface
Titer/ Working Dilution: Immunohistochemistry (Frozen and Formalin-fixed): 1-2 µg/ml
Microbiological State: This product is not sterile.