CD20 / MS4A1 (B-Cell Marker); Clone SPM618
Species: Mouse
Immunogen: Recombinant human MS4A1 protein
Clone: SPM618
Isotype: IgG2a / Kappa
Entrez Gene ID: 931
Hu Chromosome Loc.: 11q12.2
Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)
Mol. Weight of Antigen: 33-37kDa
Format: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM
PBS with 0.05% BSA & 0.05% azide.
Specificity: Recognizes a protein of 30-33kDa, which is identified as CD20.
Background: It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
Species Reactivity: Human
Positive Control: Daudi, Molt-4, Raji and U266 and human lymphocytes. Lymph nodes and tonsils.
Cellular Localization: Cell membrane
Titer/ Working Dilution: Immunohistochemistry (Frozen and Formalin-fixed): 1-2µg/ml
Flow Cytometry: 1-2µg/million cells
Immunofluorescence: 1-3µg/ml
Microbiological State: This product is not sterile.